Microsequence analysis of biologically active proteins and peptides

Microsequenceanalysis of Biologically Active Proteins and Peptides

Despite impressive advances in techniques for sequence analysis of nucleic acids, direct sequencing of proteins will continue to be required in many areas of biological research. Traditional approaches are adequate, though still laborious,when carried out on micromole (106M) amounts of material. Recent advances employing radioisotopic and carrier methodology allow sequencing in the nanomole (l0...

Biologically active peptides in milk proteins.

Bioactive peptides have been identified as digestion products of several food proteins. All the bioactive sequences are hidden in an inactive state inside the polypeptide chain of the larger protein. Milk proteins are a rich source of biologically active peptides such as exorphins (casomorphins), phosphopeptides and immunopeptides. Such peptides are released during intestinal digestion of casei...

[Biologically active peptides].

Handbook of Biologically Active Peptides

Pheromones are chemical messages known to elicit changes in behavioral reactions that are followed by physiological alterations in conspecific receiver organisms. Pheromones comprise a wide range of compounds, of varied chemical nature, that range from small-molecule volatile compounds to high-molecular-weight, water-soluble, nonvolatile peptides and proteins. Although volatile pheromone molecu...

Analysis of biologically active peptides using two-dimensional HPLC-CE.

High-performance liquid chromatography (HPLC) is a technique most frequently used for the assessment of biologically active peptides. Owing to the ionic character of these compounds, they may also be separated and assayed using capillary electrophoresis (CE), which offers very high efficiency, short analysis time and low consumption of reagents, and is used increasingly more often. The paper de...